Plant Transcription Factor Database
Previous version: v3.0
Solanum pennellii
HSF Family
Species TF ID Description
Sopen02g020980.1HSF family protein
Sopen02g023190.1HSF family protein
Sopen02g023980.1HSF family protein
Sopen02g035450.1HSF family protein
Sopen03g002000.1HSF family protein
Sopen03g004850.1HSF family protein
Sopen03g027860.2HSF family protein
Sopen04g008020.1HSF family protein
Sopen04g032370.1HSF family protein
Sopen06g029130.1HSF family protein
Sopen07g019390.1HSF family protein
Sopen07g029070.1HSF family protein
Sopen08g001190.1HSF family protein
Sopen08g018510.1HSF family protein
Sopen08g018510.3HSF family protein
Sopen08g025150.1HSF family protein
Sopen08g028860.1HSF family protein
Sopen09g022720.1HSF family protein
Sopen09g027410.1HSF family protein
Sopen09g027410.2HSF family protein
Sopen09g031520.1HSF family protein
Sopen10g030030.1HSF family protein
Sopen11g024750.1HSF family protein
Sopen12g003060.1HSF family protein
Sopen12g033300.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645